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. 2017 May 22;7:2240. doi: 10.1038/s41598-017-02472-y

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Biological activity of sea bass recombinant IL-4/13B. The expression level of genes coding for IL-4Rα1, IL-4Rα2, IL-13Rα1, IL-13Rα2, SOCS3, SAP1, HEP and IL-10 was determined in HK leukocytes after stimulation with the sea bass recombinant IL-4/13B. Transcription values were expressed as a ratio relative to 18S rRNA in the same samples. The quantitative PCR amplification was performed in PCR arrays and each point represents the mean + SD of cells from 4 individual fish. *p < 0.05 with respect to the time 0 control; **p < 0.01 with respect to the time 0 control; ***p < 0.001 with respect to the time 0 control.