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. 2017 May 22;7:2254. doi: 10.1038/s41598-017-02505-6

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Moderate controlled cortical impact mouse model. (a) The left skull was exposed and subjected to a 4-mm-diameter craniotomy using a motorized drill. Dura was intact. The craniotomy position was located in the centre of left parietal bone between bregma and the lambdoid suture. (b) The controlled cortical impact was performed using a 3-mm-diameter metal tip with a parameter of 2 mm below the dura, 3.5 m/s impact speed and 100 msec dwell time.