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. 2017 May 22;7:2215. doi: 10.1038/s41598-017-02509-2

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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5-HT₃ receptors are expressed in the lumbar dorsal horn from P7. Immunohistochemical labelling of 5-HT₃R was performed at P7 (A), P14 (B), P21 (C) and in adults (D). Scale bar = 250 μm. 5-HT₃R labelling was observed predominantly in laminae I-II in the dorsal horn from P7 and this distribution did not change with age. Quantification of 5-HT₃R intensity was performed in laminae I-II and in laminae III-V and revealed no change in 5-HT₃R intensity with age (E). Bars indicate mean ± SEM.