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. 2017 May 22;7:2238. doi: 10.1038/s41598-017-02071-x

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Over-expression of miR-125a-3p inhibits the rate of GVBD in a Fyn-dependent manner. GV oocytes were microinjected with either miR-125a-3p mimic (miR-125a-3p) or scramble-miR (Scramble), both either with or without Fyn RNA that lacks the 3′UTR region (miR-125a-3p + Fyn or, Scramble + Fyn). Oocytes were incubated for 4 hours at 37 °C in the presence of 1 µM milrinone, washed and transferred to fresh milrinone-free culture medium. Oocytes were monitored for GVBD every 30 minutes for 2.5 hours. The experiment was repeated three times. Results were analyzed by repeated measures ANOVA followed by Tukey post hoc test, and are presented in a line-chart, *p < 0.05 (A). The table (B) indicates the average of percent GVBD oocytes and SD of each dot presented at the line-chart.