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. 2017 May 22;7:2238. doi: 10.1038/s41598-017-02071-x

Figure 6.

Figure 6

miR-125a-3p decreases the interaction of A-type lamins and actin in a Fyn-dependent manner. (A,B) HEK-293 T cells, transfected with either miR-125a-3p mimic (miR-125a-3p), anti-miR or scramble-miR (Scramble), were incubated for 24 hours, lysed and subjected to (A) WB analysis using anti-Fyn antibody and anti-actin as a loading control, or to (B) immunoprecipitation with anti-lamin A/C antibody and immunoblotted with anti-actin and anti-lamin A/C. Results are normalized to scramble. Data were analyzed by one-way ANOVA followed by Tukey post hoc test. Bars are Mean ± SEM of at least three independent experiments. a,bp < 0.05; aversus Scramble; bversus miR-125a-3p. (C,D) HEK-293 T cells were transfected with Fyn-encoding vector (lacking the 3′UTR region; Fyn) or empty vector (Empty) serving as control (a); or transfected with either Fyn-encoding vector or empty vector together with either miR-125a-3p mimic (Empty + miR-125a-3p; Fyn + miR-125a-3p) or scramble-miR (Empty + scramble; Fyn + scramble) (b). Transfected cells were incubated for 24 hours, lysed and subjected to (C) WB analysis using anti-Fyn antibody and anti-actin as loading control, or to (D) immunoprecipitation with anti-lamin A/C antibody and immunoblotted with anti-actin and anti-lamin A/C. (a) Results are normalized to Empty. Data were analyzed by one-sample two-tailed student t-test. Bars are Mean ± SEM of five independent experiments, *p < 0.05. (b) Results are normalized to Empty + scramble. Data were analyzed by one-way ANOVA followed by Tukey post hoc test. Bars are Mean ± SEM of at least three independent experiments. a, bp < 0.05; aversus Empty + scramble; bversus Empty + miR-125a-3p.