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. 2017 May 22;7:2238. doi: 10.1038/s41598-017-02071-x

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fyn does not generate a complex with either A-type lamins or actin, but its activity is required for stabilization of the interaction of A-type lamins and actin. (A,B) HEK-293 T cells were transfected with either miR-125a-3p mimic (miR), anti-miR (anti) or scramble-miR (Scr), incubated for 24 hours, lysed and immunoprecipitated (IP + Ab) with (A) anti-lamin A/C antibody. The immunoprecipitated complexes were immunoblotted with anti-Fyn, anti-actin and anti-lamin A/C antibodies or immunoprecipitated with (B) anti-Fyn antibody and then immunoblotted with anti-actin and anti-Fyn antibodies. A negative control with no added antibody (IP-Ab) is displayed for each treatment. The supernatant that remained after immunoprecipitation is also displayed (SUP + Ab, SUP-Ab). (C) HEK-293 T cells were transfected with an empty-vector (Empty) or a vector encoding for dominant-negative form of Fyn (DN-Fyn), incubated for 24 hours, lysed and immunoprecipitated with anti-lamin A/C antibody and then immunoblotted with anti-actin and anti-lamin A/C antibodies. Data were analyzed by one-sample two-tailed student t-test. Bars are Mean ± SEM of three independent experiments, *p < 0.05.