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. 2017 May 22;7:2246. doi: 10.1038/s41598-017-02356-1

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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CD47 isoforms 3 and 4 are not detected at the surface erythroblast during terminal erythroid differentiation. Proerythroblasts (T0), basophilic erythroblasts (T48) and a mixture of late orthochromatic erythroblasts and reticulocytes (T144; 2 × 10⁵ cells/coverslip) were fixed in 1% PFA and 0.0075% glutaraldehyde, permeabilised in 0.05% Triton X-100, and then immunolabelled with BRIC126 (CD47 (Total)) followed by donkey anti-mouse Alexa™ Fluro 594, and CD47 isoforms 3 and 4 followed by donkey anti-rabbit Alexa™ Fluro 488. The nuclei were stained with DAPI (n = 2). Scale bar is 10 µm.