Table 1.
Summary of the proteomic profile of peptides detected following BRIC126 immunoprecipitations in K562 cells, EXP and T0 erythroblasts, and mature erythrocytes (RBCs).
| Accession Number | Protein Description | K562 cells | EXP | T0 | Mature RBCs | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Total | IgG | Ratio | Total | IgG | Ratio | Total | IgG | Ratio | Total | IgG | Ratio | ||
| P60709 | Actin, cytoplasmic 1 | 71 | 33 | 2.2 | 242 | 109 | 2.2 | ||||||
| P61158 | Actin-related protein 3 | 5 | — | * | 13 | 6 | 2.2 | ||||||
| O43707 | Alpha-actinin-4 | 8 | — | * | 59 | 20 | 3.0 | 86 | 45 | 2.0 | |||
| Q562R1 | β-actin like protein 2 | 14 | 3 | 4.7 | 45 | 19 | 2.4 | ||||||
| Q96C19 | EF hand containing protein D2 | 7 | — | * | 12 | 3 | 4 | 15 | 6 | 2.5 | 6 | — | * |
| P15311 | Ezrin | 12 | 6 | 2 | |||||||||
| P47756 | F-actin capping protein subunit β | 7 | 1 | 7 | 17 | 6 | 2.8 | ||||||
| P21333 | Filamin A | 42 | 15 | 2.8 | 54 | 6 | 9 | ||||||
| Q08722 | Leucocyte surface antigen, CD47 | 6 | 1 | 6 | 7 | 2 | 3.5 | 8 | 4 | 2 | 14 | 6 | 2.3 |
| Q9UHB6 | LIMA1 | 7 | 2 | 3.5 | 27 | 5 | 5.4 | ||||||
| P26038 | Moesin | 22 | 7 | 3.1 | 3 | 1 | 3 | ||||||
| O94832 | Myosin-Id | 12 | 2 | 6 | |||||||||
| P35579 | Myosin-9 | 228 | 72 | 3.2 | 374 | 166 | 2.3 | ||||||
| P35580 | Myosin-10 | 154 | 37 | 4.2 | |||||||||
| P12829 | Myosin light chain 4 | 13 | 2 | 6.5 | 8 | 1 | 8 | 26 | 5 | 5.2 | |||
| P35241 | Radixin | 10 | 5 | 2 | |||||||||
| P02549 | Spectrin α chain, erythrocyte | 19 | — | * | |||||||||
| P11277 | Spectrin β chain, erythrocyte | 9 | 1 | 9 | |||||||||
| Q9NYL9 | Tropomodulin-3 | 8 | — | * | 8 | 2 | 4 | 2 | 1 | 2 | |||
| P06753 | Tropomyosin α-3 chain | 9 | 1 | 9 | 3 | 3 | 1 | 5 | — | * | |||
| Q71U36 | Tubulin α-1A chain | 14 | — | * | 23 | — | * | ||||||
| P62988 | Ubiquitin | 19 | 3 | 6.3 | |||||||||
| P68036 | Ubiquitin-conjugated enzyme E2 L3 | 14 | 5 | 2.8 | 4 | * | |||||||
| P08670 | Vimentin | 5 | * | 2 | * | ||||||||
The total Bric126 IP peptides and IgG peptides are shown alongside the ratio Total/IgG. Clean total peptides are underlined and bold. *Indicates no ratio as there were no IgG immunoprecipitated peptides.