Table 6.
Prediction of conformational B-cell epitopes
| Toxin | Subunit | Residues | Score |
|---|---|---|---|
| Stx1 | A | T290, I291, S292, S293 | 0.976 |
| G25, T26, P27, L28, Q29, T30, I31, S32, S33, G34, G35, T36, S37, L39, M40, P211, D212, Y213, H214, G215, Q216, D217, S218, R220, G222, G227, S228, I229, N230, H245, A246, S247, R248, V249, A250, R251, M252, A253, S254, D255, E256, S259, P262, A263, D264, G265, R266, V267, R268, G269, I270, T271, H272, N273, K274, I275, L276, W277, S279, S280, T281, L282, G283, A284, I285, L286, M287, R288, R289 | 0.704 | ||
| F7, S8, T9, A10, K11, T12, D15, G56, I57, D58, P59, E60, E61, G62, R63, F64, N65, N66, N83, R84, T85, N86, N87, V88, Y90, R91, F92, A93, S96, H97, V98, F100, P101, G102, T103, T104, A105, V106, T107, L108, S109, G110, V120, A121, G122, I123, S124, T126, G127, M128, Q129, I130, N131, H133, T136, T137, L140, D141, M143, S144, H145, S146, G147, T148, S149, L150, T151, Q152, S153, R179, T180, T181, D183, D184, L185, S186, G187, R188, S189, Y190, V191, M192 | 0.665 | ||
| D42, S43, G44, T45, G46, D47, N48, N241, C242, H243, H244 | 0.533 | ||
| B | K33, Y34, N35, D36, D37, D38, T39 | 0.798 | |
| K3, T4, L5, I7, A8, A9, S10, L11, S12, F13, F14, S15, A16, S17, A18, L19, A20, T21, P22, T74, N75, A76, C77, H78, N79, G80, G81, G82, E85 | 0.606 | ||
| K28, V29, I65, T66, G67, M68 | 0.596 | ||
| Stx2 | A | K288, S289, Q290, F291, L292, Y293, T294, T295, G296, K297 | 0.977 |
| E29, H30, Y212, R213, G214, E215, D216, G217, R266, P267, V268, I269, K270, I271, N272, N273, T274, L275, W276, S278, N279, A281, A282, A283, F284, L285, N286, R287 | 0.771 | ||
| S8, AT9, Q10, Q11, H43, T44, P45, P46, G47, S48, G56, L57, D58, V59, Y60, Q61, A62, R63, F64, D65, H66, Q73, N74, N83, T84, A85, T86, N87, T88, Y90, R91, F92, S93, T96, H97, I98, S99, V100, P101, G102, V103, T104, T105, V106, S107, M108, T109, T110, D111, R119, V120, A121, A122, L123, E124, S126, G127, M128, Q129, I130, S131, R132, H133, V136, S137, L140, A141, M143, E144, F145, S146, G147, N148, T149, M150, T151, R152, D153, R156, Q180, A181, L182, S183, E184, T185, A186, P187, V188, T190 | 0.666 | ||
| T26, P27, L28, I31, S32, Q33, G34, T35, T36, S37, V38 | 0.610 | ||
| C260, Q261, I262, T263, G264, D265 | 0.601 | ||
| B | K12, Y13, N14, E15, D16, D17, T18, S31, R32, W33, A63 | 0.710 | |
| K7, N34, P37, Q40, S41, L44, T45, G46, M47, Q66, N68, N69, D70 | 0.700 | ||
| A1, D2, S53, S54, T55, C56, E57, S58, G59, S60 | 0.687 |