Table 2. Morphological measurements and labeling for apoptotic and synaptic markers in the cerebella of Tg(PG14) mice.
| Cerebellar area, mm2 | Granule layer width, μm | Molecular layer width, μm | TUNEL, no. of positive cells | Act. caspase-3, no. of positive cells | Synaptophysin, % positive area | |
|---|---|---|---|---|---|---|
| Tg(PG14) | ||||||
| Early (<150 days) | ||||||
| Bax+/* (n = 8) | 5.1 ± 0.5 | 90.2 ± 4.4† | 137.8 ± 6.2† | 130.6 ± 32.9† | 62.9 ± 9.8† | 50.0 ± 11.3 |
| Bax-/- (n = 6) | 7.0 ± 0.7 | 123.5 ± 7.3‡ | 154.2 ± 8.0† | 3.7 ± 0.6†‡ | 2.3 ± 0.9‡ | 54.5 ± 9.9 |
| Late (>150 days) | ||||||
| Bax+/* (n = 6) | 3.0 ± 0.4† | 55.6 ± 4.0† | 95.6 ± 6.6† | 29.0 ± 14.6 | 8.7 ± 1.6† | 32.3 ± 12.1 |
| Bax-/- (n = 3) | 4.2 ± 0.8 | 113.3 ± 8.3‡ | 92.9 ± 3.0† | 5.0 ± 2.6 | 0.7 ± 0.3‡ | 23.3 ± 8.2 |
| Tg(WT) (n = 5) | 5.9 ± 0.6 | 129.3 ± 5.3 | 180.0 ± 7.2 | 1.6 ± 0.5 | 0.0 ± 0.0 | 61.2 ± 15.1 |
Entries show the mean value ± SEM. The number of animals in each group is given in parentheses. Bax+/* refers to mice that were either Bax+/- or Bax+/+. Values for Tg(WT) mice at different ages (62-451 days old) were similar and were therefore pooled. Act., activated.
†
Significantly different from the corresponding value for Tg(WT) (P < 0.05), two-tailed t test with Satterthwaite correction
‡
Significantly different from the corresponding value for Tg(PG14)/Bax+/* (P < 0.01), two-tailed t test with Satterthwaite correction