Skip to main content
. 2017 May 23;7:202. doi: 10.3389/fcimb.2017.00202

Table 1.

Primers used in this study.

Primer Sequence (5′→3′)* Purpose
mubKupF GAGTATATCATTCAATATATCCTG Comparison of IFDC2 with ePheS variants to create IFDC3
mubKupR TGCTATGAGTGTTATTGTTGCTCGGCATCCCTCCCACCTAAATATGATTC
mubKdnF TATTAGGTATACTACTGACAGCTTCTGAAATGAGTGAAAAGGAAATGTCA
mubKdnR GACTCGAATTCCATGTGTTTTCTCC
IFDCF CCGAGCAACAATAACACTCATAGCA
IFDCR GAAGCTGTCAGTAGTATACCTAATA
IFDC3-T260SF GCGTCCTTCTTATTTCCCATTTTCTGAGCCTTCTGTTGAAGTCGATG
IFDC3-T260SR CATCGACTTCAACAGAAGGCTCAGAAAATGGGAAATAAGAAGGACGC
IFDC3-T260AF GCGTCCTTCTTATTTCCCATTTGCTGAGCCTTCTGTTGAAGTCGATG
IFDC3-T260AR CATCGACTTCAACAGAAGGCTCAGCAAATGGGAAATAAGAAGGACGC
mub156dnF GAATCATATTTAGGTGGGAGGGATGGGGGCCGCAGAAGCGGAAGGATTAAAAG Comparison of different direct repeat lengths for deleting 156 bp within the mubK gene of the mutanobactin gene locus
mub156dnR TACTAAATGGTAGCCAATTTGATAG
DR-R CATCCCTCCCACCTAAATATGATTC
DR25IFDC-R CATCCCTCCCACCTAAATATGATTCGAAGCTGTCAGTAGTATACCTAATA
DR100-F TATTAGGTATACTACTGACAGCTTCCATAAAGTAAGTTCTATTTATTCTG
DR200-F TATTAGGTATACTACTGACAGCTTCTGGTTGCTTAATGGAATTTCTTGAA
DR400-F TATTAGGTATACTACTGACAGCTTCACCAGTTGCTCGCGAACCACCAAGA
mub625dnF GAATCATATTTAGGTGGGAGGGATGTTGTCTTGTCTTTAGTAGCTGGCAT Comparison of different deletion lengths within the mutanobactin gene locus
mub2.5dnF GAATCATATTTAGGTGGGAGGGATGAGAAAACACATGGAATTCGAGTCAA
mub10dnF GAATCATATTTAGGTGGGAGGGATGGTCAAAAGTTTTGACCATGTTGTTCT
mub4dnF GAATCATATTTAGGTGGGAGGGATGAATAACTATGGGAAGCCAAGAGTAA
ComYATAAupF CCAGGCATTCTTATGGATATTGCCG Creation of markerless comYA-TAA nonsense mutation
ComYATAAupR TGCTATGAGTGTTATTGTTGCTCGGTTAAACCATAAAACCTCCCATCTTATC
ComYATAADR-F TATTAGGTATACTACTGACAGCTTCTGATACCCCTTTTCGTTCCAAATG
ComYATAADR-R CGGCAATGATTTTTCTACCTAATTTTTAAACCATAAAACCTCCCATCTTATC
ComYATAAdnF AAATTAGGTAGAAAAATCATTGCCG
ComYATAAdnR CTATAATTTCTGTTAAATTAAAACC
ComYATAGDR-R CGGCAATGATTTTTCTACCTAATTTCTAAACCATAAAACCTCCCATCTTATC Creation of markerless comYA-TAG nonsense mutation
ComYATAGupR TGCTATGAGTGTTATTGTTGCTCGGCTAAACCATAAAACCTCCCATCTTATC
ComYATGADR-R CGGCAATGATTTTTCTACCTAATTTTCAAACCATAAAACCTCCCATCTTATC Creation of markerless comYA-TGA nonsense mutation
ComYATGAupR TGCTATGAGTGTTATTGTTGCTCGGTCAAACCATAAAACCTCCCATCTTATC
comYBTAAupF GAGAATAGGGAATGAAAGGAGGTTC Creation of markerless comYB-TAA nonsense mutation
ComYBTAAupR GCTATGAGTGTTATTGTTGCTCGGTTATCGCATTTTCTTTGTGTATATTTC
comYBTAADR-F TATTAGGTATACTACTGACAGCTTCGTGGAACAAACAAATTTATGACCTT
comYBTAADR-R CCAAATGCAGACCATTCAACAACCCTTATCGCATTTTCTTTGTGTATATTTC
ComYBTAAdnF TAAGGGTTGTTGAATGGTCTGCATTTG
comYBTAAdnR CTTTAACCACGGCAGAACCGCCAG
comYCupF CACTGAAGGAGAAGCACAAGCAG Creation of markerless comYC-TAA nonsense mutation
ComYCTAADR-R CGTACACTGACTCTCTTGATCTTTTACATTATAAATTAACCTCCATATTCTG
comYCTAAdnF TAAAAGATCAAGAGAGTCAGTGTACG
ComYCTAAupR CTATGAGTGTTATTGTTGCTCGGTTACATTATAAATTAACCTCCATATTCTG
comYCDR-F TATTAGGTATACTACTGACAGCTTCAATATGGAGAAGTAAAGTCAAAATTG
comYCTAAdnR CAGATACACTACCAGCAATGACAAG
*

Complementary sequences used for OE-PCR are shown in bold, while mutagenic sequences used to introduce missense and nonsense mutations are underlined in italics.