Fig. 5.

The levels of IFN-γ-expressing, TNF-α-expressing, and IL-17-expressing CD4+ Tem cells are decreased in the blood of NSCLC patients. a IFN-γ, TNF-α, and IL-17 production by the CD4+ and CD8+ Tem cells in the blood and lymph node, gated on the unstimulated controls. The numbers in the quadrants indicate the percent of the CD4+ T cells (left) or CD8+ T cells (right) that produce IFN-γ, TNF-α, IL-17, or both cytokines. b The mean frequency (±SEM) of the IFN-γ-expressing, TNF-α-expressing, and IL-17-expressing T cells gated on the CD4+ Tem (left) and CD8+ Tem (right) T cells in the blood. *p < 0.05, **p < 0.005, ***p < 0.001; Mann–Whitney test (two-tailed) and non-paired Student’s t test. c The plot of IFN-γ-secreting and TNF-α-secreting CD4+ Tem and CD8+ Tem cells in the blood and lymph node from non-small cell lung cancer patients and healthy donors, expressed as the mean ± SEM