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Relative gene expression in hMSCs cultured on scaffolds in the absence of chondrogenic induction. Gene expression is normalized to the starting hMSCs (set as 1) and the housekeeping gene GAPDH. The ratio of COL2A1/COL1A1 (c) was calculated from the relative expressions of COL1A1 (a) and COL2A1 (b) as described in Materials and Methods. Data shown are a representative of two independent experiments. Data are presented as mean ± SD (n = 3, **p < 0.01)
