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. 2004 Dec 23;102(1):93–98. doi: 10.1073/pnas.0408455102

Fig. 3.

Fig. 3.

Expression of the melanogenesis regulators in neonatal and adult skin of WT and K5-Noggin mice. Dorsal skin of WT and TG mice harvested at P2.5 or 5 days after depilation was processed for detection of the ASP, MC-1R, attractin, α-MSH, SCF, and M-MITF expressions by RT-PCR, Western blot analysis, and in situ hybridization. (A) RT-PCR of ASP, MC-1R, attractin, M-MITF, and β-actin in skin of WT and TG mice. Representative gels from one of three experiments each are shown. (B) Western blot analysis of β-actin, ASP, α-MSH, MITF, and SCF proteins in skin lysates of WT and TG mice. (C) Expression of the ASP transcripts in the outer (small arrowheads) and inner (large arrowheads) root sheaths, dermal papilla (large arrows), and hair matrix (small arrow) of anagen HF in WT mice. (Inset) High magnification of the labeled area. (D) Decrease of the ASP mRNA expression in the HF epithelium (arrowhead) and dermal papilla (arrow) of anagen HF in TG mice. (Inset) High magnification of the labeled area. (E) Absence of the ASP mRNA in anagen HF of C57BL/6 mice used as a negative control for ASP expression.