Figure 2.

GRP78 is bound (4°C) but not endocytosed (37°C) by dendritic cells. DC2.4 cells (A,B) or bone marrow-derived dendritic cells (C,D) were incubated with AF488-GRP78 or BSA (1 µM) at 4°C for 1 h. Fluorescence was detected by flow cytometry (FCM) (A,C) or confocal laser scanning microscopy (CLSM) (B,D). (E) DC2.4 cells were stained with AF488-GRP78 or BSA at 37°C for 1 h. The location of GRP78 was detected by CLSM. Error bars represent mean ± SD from triplicate samples in one experiment. *P < 0.05 and ***P < 0.001. All images for all panels are representative of at least three independent experiments in which >100 cells were examined and >95% of cells showed similar staining. Scale bar, 20 µm.