Figure 5.

PRMT5 knockdown in T cells reveals increased sensitivity to TGF-β. CD4⁺CD25⁻ T cells were transfected with shRNA that targeted PRMT5 or with control shRNA. (A) After 48 h, the PRMT5 expression was measured with Western blot analysis. (B) The transfected cells were or were not differentiated under Treg-promoting conditions and were analyzed at day 3 for Foxp3 expression with flow cytometry. The numbers represent the CD4⁺Foxp3⁺ cell frequency. (C) CD4⁺CD25⁻ T cells were transfected with PRMT5 shRNA, and they were differentiated under Treg-promoting conditions in the presence of a TGF-β signaling inhibitor (LY364947, 1 and 5 µM). The data shown are representative of at least three independent experiments. The P values represent significant differences between the LY364947 and medium control-treated cells. *P < 0.05, **P < 0.01.