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. 2017 May 23;10:153. doi: 10.3389/fnmol.2017.00153

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Copyright © 2017 Bronstein, Kyle, Abraham and Tsirka.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Ratios of neural and glial cells altered in HMGB2^-/- SVZ cultures at day 3 of differentiation. NSPCs were cultured in eight-well chamber slides with growth factor-free media and allowed to differentiate. Chamber slides from HMGB2^+/+ and HMGB2^-/- NPCs stained for the immature neuronal marker Tuj1, all results represented as percentage of Tuj1⁺ (red) cells to all 4′,6-diamidino-2-phenylindole⁺ (DAPI⁺) (blue)nuclei. Chamber slides from HMGB2^+/+ and HMGB2^-/- NPCs stained for the astrocyte marker GFAP, all results represented as percentage of GFAP⁺ (red) cells to all DAPI⁺ (blue) nuclei. n = 3 biological replicates per genotype. Sixty cells were counted per biological replicate. ^∗∗GFAP p = 0.0049. Scale bar = 150 μm.