Table 1. Production of cloned mouse by using conventional methods.
No. of embryos reaching, %
|
No. (%) of fetuses at 19.5 dpc‡
|
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---|---|---|---|---|---|---|---|---|
Mouse strain | Type of donor cell | Gender | No. of successfully reconstructed oocytes* | Two to eight cells | Morulae/blastocysts | No. of morulae/blastocysts transferred (recipients) | Aborted fetuses or placentae only | Live offspring |
ICR hermaphrodite | Tail tip | Male? | 75 | - | 18 (24) | 18 (1) | 9 | 0 |
ICR wild type | Tail tip | Male | 230 | - | 24 (10.4) | 24 (3) | 0 | 0 |
Tail tip | Female | 160 | - | 7 (4.4) | 7 (1) | 0 | 0 | |
B6D2 F1 | Cumulus | Female | 420 | 259 | - | 156 (13)† | 1 | 4 (2.6) |
154 | - | 73 (47.4) | 73 (5) | 0 | 2 (2.7) |
Survived nuclear injection
Transferred at two- to eight-cell stage
Days postcopulation