Table 2. Establishment of ntES cell lines by nuclear transfer from various mouse strains.
Mouse strain | Cell type | Gender | No. of reconstructed oocytes | No. of morulae/blastocyst produced from oocytes, % | No. of established ntES cell lines from embryos, % |
---|---|---|---|---|---|
ICR hermaphrodite | Tail tip | Male? | 100 | 31 (31) | 11 (36) |
ICR wild type | Tail tip | Male | 114 | 11 (10) | 1 (9) |
Tail tip | Female | 136 | 13 (10) | 3 (23) | |
B6D2 F1 | Cumulus | Female | 88 | 49 (56) | 8 (16) |