Table 4. ntES cell contribution to chimeras after cell injection into normal fertilization-derived blastocysts.
| No. of live offspring
|
Germ-line transmission
|
||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Origin of ntES cell line
|
Recipient blastocyst
|
Chimeric contribution in coat color (F/M)†
|
|||||||||
| Strain | Cell type | Strain | Ploidy | No. of blastocysts transferred (no. recipients) | Total | Total no. of chimeras | High‡ (>50%) | Low‡ (<50%) | Mated with ICR female or male | Proved to be infertile | Proved to be transmitted ntES genes |
| ICR hermaphrodite | Tail | B6D2F2 | 2n | 102 (7) | 42 + 29* | 25 (10/15) | 10 (3/7) | 15 (7/8) | 10 | 2 | 1 |
| 4n | 52 (4) | 2 | 2 (0/2) | 2 (0/2) | 0 | 2 | 2 | 0 | |||
| B6D2 F1 | Cumulus | ICR | 2n | 16 (1) | 12 | 8 (4/4) | 4 (3/1) | 4 (1/3) | 3 | 0 | 3 |
*
Died soon after birth
†
F, female; M, male
‡
Contribution was scored either high (>50% of coat color was derived from ntES cells) or low (<50%)