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. 2017 May 16;37(11):e00079-17. doi: 10.1128/MCB.00079-17

FIG 3.

FIG 3

IRP2 is a succination target. (A) Multiple-sequence alignment of mouse Aco2, human IRP2 (ACO3), and IRP1 (ACO1). Succination sites reported for Fh−/− mouse Aco2 are conserved in human IRP1 and IRP2. These sites are highlighted in red. (B) Adduct sites on Flag-tagged wild-type IRP2, as determined by LC-MS/MS. Black bars represent peptides detected by mass spectrometry analysis. (C) Adduct sites on Flag-tagged IRP2-A523R, as determined by LC-MS/MS. Black bars represent peptides detected by mass spectrometry analysis.