FIG 7.

Overexpressed Fes1 and Snl1ΔN do not replace the function of Sse1 and Sse2 in the reactivation of heat-aggregated firefly luciferase. (A) Western analysis of sse1-200 sse2Δ cells transformed with an empty vector control (−) or plasmids that express Sse1 or Fes1 at lower (Fes1⁺) or higher (Fes1⁺⁺⁺) levels. Fes1 expression levels were determined relative to those of the strain transformed with the Sse1-expressing plasmid (bottom). B) Growth of sse1-200 sse2Δ cells transformed with an empty plasmid vector (−) or derivatives that express Sse1, Fes1⁺, or Fes1⁺⁺⁺. (C) Reactivation of heat-aggregated cytosolic firefly luciferase (FFL-GFP-NES) in Fes1- and Snl1ΔN-overexpressing strains was monitored by bioluminescence measurements. Error bars represent standard errors of data from triplicate experiments.