Figure 3. Time-dependent exchange experiment.

Percentage of C₁₂BTA3D as a function of time after the dilution of a 500 μM BTA solution 100 times into D₂O, at room temperature. The black line is a tri-exponential fit of the experimental data including the point (t=0 h, C₁₂BTA3D=100%), with k_initial=1.8 × 10¹ h⁻¹, k_fast=1.4 × 10⁻¹ h⁻¹ and k_slow=0.7 × 10⁻² h⁻¹. The error bars represent one s.d. of uncertainty computed from three separate kinetic experiments. The inset shows an enlargement of the data points and fit for the first 5 h.