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. 2017 May 15;8:15014. doi: 10.1038/ncomms15014

Figure 3. Change in tissue shape induces alternative configuration of the ROCK-myosin signalling module.

Figure 3

(a) Apical myosin meshwork (green) organized into a-p oriented fibres during VF formation in Ctr-RNAi embryos (arrows) but organized into rings in Spn27A-RNAi and Fat2-RNAi embryos (arrows). Red arrowheads point to radially oriented spokes that connected myosin rings from cell to cell. a, anterior; p, posterior. (b) Images of VF cells in fixed wild-type and Spn27A-RNAi embryos showing the localization of ROCK::GFP (green) sqh::mCherry (blue) and MBS (red). Myosin and MBS formed a fibrous network and ROCK a condensed focus in wild-type cells (arrow), whereas Myosin, MBS and ROCK organized into rings in Spn27A-RNAi cells (arrow). (c,d) Quantification of ROCK, myosin and MBS polarization in the apical domain of VF cells as the signal intensity profile from the centre of ROCK density to the apical margin. Left, schematic of cells with radial lines emanating from the ROCK centre of mass. Right, mean normalized intensity profile of ROCK, myosin and MBS in wild-type cells (c, n=152 cells, one embryo) and Spn27A-RNAi cells with clear ROCK rings (d, n=27 cells, one embryo). Shaded area indicates s.d. Scale bars, 5 μm (a) and 10 μm (b).