Table 1. Binding affinity of C05 variants against hemagglutinins from different influenza strains.
| Kd in nM | Residue 190 | VVSAGW (WT) | VPGSGW | VVSSGW | VTGSGW |
|---|---|---|---|---|---|
| A/Solomon Islands/3/2006 (H1N1) | D | 91.4±0.9 | 15.0±0.2 | 4.6±0.6 | 115.8±1.9 |
| A/New Caledonia/20/1999 (H1N1) | N | 6.3±0.1 | 0.6±0.0 | 2.9±0.1 | 1.1±0.2 |
| A/Beijing/262/1995 (H1N1) | V | 23.1±0.6 | 12.6±0.7 | 259.3±2.8 | 182.3±0.5 |
| A/WSN/1933 (H1N1) | E | 169.4±1.0 | 719.6±2.9 | 727.7±20.5 | 1,539±42 |
| A/Japan/305/1957 (H2N2) | E | 1,701±46 | >5,000 | >5,000 | >5,000 |
| A/Hong Kong/1/1968 (H3N2) | E | 327.8±12.0 | 958.8±45.6 | 1,100±94 | 1,860±83 |
| A/Panama/2007/1999 (H3N2) | D | 926.3±12.1 | 1,306±12 | 1,290±71 | 4,335±207 |
| A/Perth/16/2009 (H3N2)* | D | 23.4±0.1 | 43.6±1.0 | 43.7±0.5 | 125.1±1.2 |
*C05 is able to make additional interaction with A/Perth/16/2009 (H3N2) via CDR H1 (ref. 21).