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Hemolytic activity of P. damselae subsp. damselae plasmidless strain AVL49 and mutant derivatives on TSA-1 plates supplemented with 5% sheep or fish (trout) erythrocyte suspensions, either in the presence or absence of lecithin (supplemented with 5% egg yolk extract). hlyAch and plpV are the chromosome I-borne genes encoding PhlyC hemolysin and PlpV phospholipase, respectively. Bar, 1 cm.
