TABLE 2.
Results of the PCR screening for gene markers and of phenotypic tests for the 34 Photobacterium damselae subsp. damselae strains used in this study
| Strain | Source | pPHDD1a | hlyAch | Caseinase | Gelatinase |
Phospholipase |
||
|---|---|---|---|---|---|---|---|---|
| Testb | colP | Testc | plpV | |||||
| RM-71 | Turbot, Spain | + | + | − | − | − | ++ | + |
| RG-153 | Turbot, Spain | + | + | − | − | − | ++ | + |
| RG-91 | Turbot, Spain | + | + | − | − | − | ++ | + |
| RG-214 | Turbot, Spain | + | + | − | − | − | ++ | + |
| AZ 245.1 | Turbot, Spain | + | + | − | − | − | ++ | + |
| CDC-2227-81 | Human, United States | + | + | − | − | − | ++ | + |
| CIP 102761 | Damselfish, United States | + | + | − | − | − | ++ | + |
| ATCC 35083 | Brown shark, United States | − | + | − | − | − | + | + |
| 9FT1M-3 | Shark, United States | − | + | − | + | + | + | + |
| 9FT2B-2 | Shark, United States | − | + | − | + | + | + | + |
| RS80L1V1 | Red snapper, United States | − | + | − | − | − | + | + |
| RS78SPL1 | Red snapper, United States | − | + | − | − | − | + | + |
| ST-1 | Sea trout, United States | − | + | − | − | − | + | + |
| A-162 | Eel, Belgium | − | + | − | + | + | + | + |
| 158 | Eel, Belgium | − | + | − | + | + | + | + |
| PG-801 | Shrimp, Taiwan | − | + | − | − | − | + | + |
| J3G-801 | Shrimp, Taiwan | − | − | − | − | − | + | + |
| 309 | Mussel, Spain | − | + | − | − | − | + | + |
| 192 | Dolphin, United States | − | + | − | − | − | + | + |
| 238 | Dolphin, United States | − | + | − | − | − | + | + |
| AZ 247.1 | Turbot, Spain | − | + | − | − | − | + | + |
| ACR 208.1 | Turbot, Spain | − | + | − | − | − | + | + |
| USC-Viro-1 | Turbot, Spain | − | + | − | − | − | + | + |
| ACRP-72.1 | Turbot, Spain | − | − | − | − | − | + | + |
| LD-07 | Gilthead seabream, Spain | − | + | − | + | + | + | + |
| TW 294 L2 | European sea bass, Spain | − | + | − | − | + | + | |
| TW 250/03 | Gilthead seabream, Spain | − | + | − | + | + | + | + |
| TW 462/02.1 | Gilthead seabream, Spain | − | + | − | − | − | + | + |
| DCL 1.2 | Seabream, Spain | − | + | − | − | − | + | + |
| DCL 4.1 | Seabream, Spain | + | + | − | − | − | ++ | + |
| DCL 7.1 | Seabream, Spain | + | + | − | + | + | ++ | + |
| DCL 7.3 | Seabream, Spain | + | + | − | − | − | ++ | + |
| DCL 8.1 | Seabream, Spain | − | + | − | + | + | + | + |
| DCL 9.1 | Seabream, Spain | − | + | − | − | − | + | + |
a
pPHDD1 presence was assessed by PCR targeted to the damselysin (dly) gene.
b
Gelatinase agar plate test with 1% gelatin.
c
Phospholipase agar plate test with 3% egg yolk. +, medium halo (strains that only produce PlpV); ++, large halo (strains that produce Dly and PlpV).