TABLE 3.
Strains and plasmid used and constructed in this study
| Strain or plasmid | Descriptiona | Reference or source |
|---|---|---|
| Strains | ||
| P. damselae subsp. damselae | ||
| RM-71 | Isolated from turbot; pPHDD1 | 4 |
| AR57 | RM-71 derivative, spontaneous rifampin-resistant mutant, Rfr | 9 |
| AR78 | AR57 with in-frame deletion of dly and hlyApl, Rfr | 9 |
| AR64 | AR57 with in-frame deletion of dly, Rfr | 9 |
| AVL64 | AR57 with in-frame deletion of plpV, Rfr | This study |
| AVL66 | AR57 with in-frame deletion of dly and plpV, Rfr | This study |
| A-162 | Isolated from eel (Anguilla anguilla) in Belgium, 1991 | Laboratory collection |
| LD-07 | Isolated from gilthead seabream, plasmidless strain | 5 |
| AVL49 | LD-07, spontaneous rifampin-resistant mutant, Rfr | This study |
| AVL63 | AVL49 with in-frame deletion of hlyAch, Rfr | This study |
| AVL58 | AVL49 with in-frame deletion of plpV, Rfr | This study |
| AVL60 | AVL49 with in-frame deletion of colP, Rfr | This study |
| AVL62 | AVL49 with in-frame deletion of hlyAch and plpV, Rfr | This study |
| AVL68 | AVL49 with in-frame deletion of hlyAch, plpV, and colP, Rfr | This study |
| AR211 | LD-07 derivative with in-frame deletion of epsL, Rfr | 17 |
| AR240 | LD-07 derivative with in-frame deletion of pilD, Rfr | 17 |
| E. coli | ||
| DH5α | Cloning strain | Laboratory stock |
| S17-1 λpir | RP4-2(Km::Tn7, Tc::Mu-1) pro-82 λpir recA1 endA1 thiE1 hsdR17 creC510 | 47 |
| Plasmid | ||
| pNidKan | Suicide vector derived from pCVD442, Kmr | 48 |
a
Rfr, rifampin resistance; Kmr, kanamycin resistance.