TABLE 1.
Characterization of the microbial communities of leaf and root samples from 73 citrus trees across Floridaa
| Location | Citrus tree species and cultivar | Date(s) surveyed (mo/day/yr) | No. of: |
HLB symptom appearance | ||
|---|---|---|---|---|---|---|
| Trees | Leaf communities | Root communities | ||||
| Gainesville, FL | Citrus sinensis L. Osbeck cv. Valencia | 4/1/15, 6/1/15, 9/22/15, 3/29/16 | 8b | 8/date | 8/datec | IV |
| Fort Pierce, FL | Citrus sinensis L. Osbeck cv. Valencia | 10/13/15 | 15 | 14 | 9 | III |
| Immokalee, FL | Citrus sinensis L. Osbeck cv. Valencia | 11/3/15 | 5 | 5 | 0 | III |
| Citrus paradisi Macfadyen cv. Ray Ruby | 11/3/15 | 5 | 5 | 5 | IV | |
| Citrus sinensis (L.) Osbeck cv. Navel | 11/3/15 | 5 | 5 | 5 | II | |
| Quincy, FL | Citrus sinensis (L.) Osbeck cv. Navel | 10/20/15 | 10 | 9 | 10 | I |
| Citrus unshiu Marcovitch cv. Owari | 10/20/15 | 5 | 5 | 5 | I | |
| Citrus × tangelo cv. Honeybell | 10/20/15 | 3 | 3 | 3 | I | |
| Vero Beach, FL | Citrus sinensis L. Osbeck cv. Valencia | 11/2/15 | 5 | 4 | 2 | II |
| Citrus paradisi Macfadyen cv. Ray Ruby | 11/2/15 | 12 | 12 | 9 | I | |
a
For each sample set, the location, citrus cultivar, sampling date, number of trees sampled, numbers of leaf and root microbial communities included in data analyses (i.e., the subset of total samples that passed quality control and contained >1,000 16S sequences with assigned taxonomy), and huanglongbing (HLB) symptom appearance are listed. For the latter, the categories I, II, III, and IV correspond to asymptomatic, symptomatic—mild, symptomatic—moderate, or symptomatic—severe, respectively. (See Fig. S2 for detailed description.)
b
The same 8 trees were sampled on each date.
c
On 6/1/15, only 7 replicates were included in the analysis.