TABLE 1.
Characteristics of streptococcal isolatesa
| HOMD ID | Subject 1 |
Subject 2 |
||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| No. of clones | RS ID no. | RPS type | No. of coaggregation partners |
No. of clones | RS ID no. | RPS type | No. of coaggregation partners |
|||||
| Streptococci | Others | Total | Streptococci | Others | Total | |||||||
| Streptococcus anginosus HOT 543 | 4 | 1 | 0 | 0 | 0 | No isolates | ||||||
| Streptococcus australis HOT 073 | No isolates | 1 | 1 | 3 | 10 | 13 | ||||||
| 1 | 2 | 3G(w) | 1 | 11 | 12 | |||||||
| 1 | 3 | 3G | 3 | 12 | 15 | |||||||
| 1 | 4 | 3G | 2 | 14 | 16 | |||||||
| 1 | 5 | 3 | 10 | 11 | 21 | |||||||
| 1 | 6 | 3G | 4 | 11 | 15 | |||||||
| Streptococcus dentisani HOT 398 | No isolates | 1 | 20 | 0 | 1 | 1 | ||||||
| 1 | 21 | 12 | 11 | 23 | ||||||||
| 3 | 22 | 0 | 0 | 0 | ||||||||
| 1 | 23 | 3(w) | 0 | 0 | 0 | |||||||
| Streptococcus gordonii HOT 622 | 1 | 2 | 1Gn | 0 | 6 | 6 | No isolates | |||||
| Streptococcus infantis HOT 638 | 2 | 3 | 5 | 2 | 7 | 1 | 7 | 2 | 12 | 14 | ||
| 2 | 4 | 4(w) | 3 | 2 | 5 | 1 | 8 | 6 | 11 | 17 | ||
| 1 | 9 | 5 | 11 | 16 | ||||||||
| 1 | 10 | 4 | 4 | 7 | 11 | |||||||
| Streptococcus mitis HOT 677 | 1 | 5 | 3 | 0 | 0 | 0 | 7 | 11 | 4(w) | 1 | 3 | 4 |
| 3 | 6 | 3 | 3 | 3 | 6 | 2 | 12 | 0 | 2 | 2 | ||
| 1 | 7 | 4 | 0 | 1 | 1 | 3 | 13 | 3Gn | 1 | 11 | 12 | |
| 1 | 8 | 2 | 2 | 4 | 3 | 14 | 0 | 8 | 8 | |||
| 3 | 9 | 1Gn | 1 | 6 | 7 | 1 | 15 | 0 | 1 | 1 | ||
| 4 | 10 | 1Gn | 1 | 6 | 7 | 1 | 16 | 0 | 2 | 2 | ||
| 1 | 11 | 0 | 0 | 0 | 1 | 17 | 0 | 6 | 6 | |||
| 1 | 12 | 1Gn | 1 | 6 | 7 | 1 | 18 | 0 | 0 | 0 | ||
| 2 | 13 | 1 | 2 | 3 | 1 | 19 | 3Gn | 1 | 8 | 9 | ||
| 1 | 14 | 1 | 0 | 1 | ||||||||
| Streptococcus oralis HOT 707 | No isolates | 1 | 24 | 6 | 14 | 20 | ||||||
| 1 | 25 | 7 | 15 | 22 | ||||||||
| Streptococcus parasanguinis II HOT 411 | 4 | 15 | 2 | 2 | 4 | 1 | 26 | 0 | 5 | 5 | ||
| 2 | 16 | 0 | 1 | 1 | 1 | 27 | 0 | 1 | 1 | |||
| 1 | 17 | nt | nt | nt | 1 | 28 | 2 | 4 | 6 | |||
| 1 | 18 | nt | nt | nt | 1 | 29 | 10 | 3 | 13 | |||
| 1 | 19 | 2 | 2 | 4 | ||||||||
| Streptococcus parasanguinis I HOT 721 | No isolates | 1 | 30 | 0 | 1 | 1 | ||||||
| Streptococcus salivarius HOT 755 | 4 | 20 | 0 | 1 | 1 | 9 | 31 | 1 | 0 | 1 | ||
| 2 | 21 | 0 | 0 | 0 | ||||||||
| Streptococcus salivarius/vestibularis | 3 | 22 | 3 | 5 | 4 | 9 | 1 | 32 | 3 | 5 | 8 | 13 |
| 2 | 23 | 0 | 2 | 2 | 5 | 33 | 3 | 8 | 8 | 16 | ||
| 7 | 24 | 6 | 5 | 11 | 2 | 34 | 3 | 6 | 8 | 14 | ||
| 1 | 25 | 0 | 2 | 2 | 2 | 35 | 3(w) | 8 | 8 | 16 | ||
| 1 | 36 | 1 | 0 | 1 | ||||||||
| 2 | 37 | 1 | 1 | 2 | ||||||||
| Streptococcus sanguinis HOT 758 | 2 | 26 | 3G | 5 | 8 | 13 | 5 | 38 | 0 | 3 | 3 | |
| 2 | 39 | 0 | 2 | 2 | ||||||||
| 2 | 40 | 1Gn | 0 | 7 | 7 | |||||||
| 2 | 41 | 2 | 5 | 7 | ||||||||
| Streptococcus sp. strain HOT 061 | No isolates | 1 | 42 | 3(w) | 2 | 10 | 12 | |||||
| 1 | 43 | 3(w) | 2 | 9 | 11 | |||||||
| Streptococcus sp. strain HOT 064 | 11 | 27 | 4Gn | 5 | 6 | 11 | No isolates | |||||
| 2 | 28 | 0 | 2 | 2 | ||||||||
| Streptococcus sp. strain HOT 431 (previously uncultivated) | No isolates | 4 | 44 | 7 | 2 | 9 | ||||||
| 1 | 45 | 4 | 0 | 2 | 2 | |||||||
| 1 | 46 | 0 | 1 | 1 | ||||||||
| 1 | 47 | 9 | 2 | 11 | ||||||||
| Streptococcus tigurinus HOT 071 | 1 | 29 | H1 | 0 | 0 | 0 | No isolates | |||||
| 3 | 30 | 0 | 0 | 0 | ||||||||
| Streptococcus vestibularis HOT 021 | 2 | 31 | 1 | 2 | 3 | 1 | 48 | nt | nt | nt | ||
| 1 | 32 | 0 | 0 | 0 | 1 | 49 | nt | nt | nt | |||
| 2 | 33 | nt | nt | nt | ||||||||
| 2 | 34 | 0 | 0 | 0 | ||||||||
| Avg/median | 1.3/1 | 2.4/2 | 3.8/3 | 2.8/1 | 5.3/5 | 8.6/9 | ||||||
The number of clones in each REP-PCR pattern for the isolates of each HOT, the identifier (ID) number of the representative strain (RS), the type of RPS (if present), and the number of coaggregations for each RS with coisolated streptococcal and nonstreptococcal RSs are listed. For example, 4 isolates of S. infantis HOT 638 were obtained from subject 2 which displayed 2 distinct REP-PCR patterns, with 2 isolates falling into each pattern. One isolate from each pattern was designated a representative strain, and those isolates were given strain-representative strain numbers 3 and 4. Strain 4 reacted weakly (w) with anti-serotype-4 antibody, but a recognition motif could not be assigned. This strain coaggregated with 3 other coisolated streptococcal RSs and 2 nonstreptococcal RSs. nt, not tested.