TABLE 1.
Inhibition of human and/or fish pathogens by three Pseudoalteromonas piscicida strains, DE1-A, DE2-A, and DE2-B, in triplicate assays
| Pathogen | Zone of clearing (mm) around colonies of P. piscicidaa: |
||
|---|---|---|---|
| DE1-A | DE2-A | DE2-B | |
| Marine bacteria | |||
| Aeromonas hydrophila | 0.5 | <0.5 | 1.5 |
| 0.5 | 0 | 1 | |
| 0.5 | 0 | 0 | |
| Listonella anguillarum | 0.5 | 0.5 | 0.5 |
| 0.5 | 0.5 | 0.5 | |
| 0.5 | 0.5 | 0.5 | |
| Photobacterium damselae | 0.5 | 2 | 2.5 |
| 0.5 | 2 | 2.5 | |
| 0.5 | 1 | 2 | |
| Shewanella algae | 1 | 0.5 | 0.5 |
| 1 | 0.5 | 0.5 | |
| 1 | 0.5 | 0.5 | |
| Vibrio alginolyticus | 0.5 | 0 | 0 |
| <0.5b | 0 | 0 | |
| 0 | 0 | 0 | |
| Vibrio cholerae | 1 | 1 | 1 |
| 1 | 1 | 1 | |
| 1 | 1 | 1 | |
| Vibrio coralliilyticus | <0.5 | <0.5 | <0.5 |
| <0.5 | <0.5 | <0.5 | |
| <0.5 | <0.5 | <0.5 | |
| Vibrio parahaemolyticus O3:K6 | 1 | 1.5 | 1 |
| 1 | 1.5 | 0.5 | |
| 1 | 1 | 0.5 | |
| Vibrio vulnificus MLT362 | 3.5 | 3 | 3 |
| 3.5 | 3 | 3 | |
| 3.5 | 3 | 2.5 | |
| Vibrio vulnificus MLT364 | 2.5 | 2.5 | 2 |
| 2.5 | 2 | 2 | |
| 2 | 2 | 1.5 | |
| Vibrio vulnificus MLT1003 | 1.5 | 1 | 1.5 |
| 1.5 | 1 | 1.5 | |
| 1 | 1 | 1 | |
| Anthropogenic bacteria | |||
| Escherichia coli O157:H7 | 0 | 0 | 0 |
| 0 | 0 | 0 | |
| 0 | 0 | 0 | |
| Salmonella enterica serovar Typhimurium | 0 | 0 | 0 |
| 0 | 0 | 0 | |
| 0 | 0 | 0 | |
| Shigella sonnei | 1.5 | 0 | 0 |
| 1.5 | 0 | 0 | |
| 1.5 | 0 | 0 | |
| Staphylococcus aureus | 1 | 1 | 1 |
| 1 | 1 | 1 | |
| 0.5 | 1 | 0 | |
Zones of clearing were measured around 48-h-old colonies of three P. piscicida isolates grown at 24 to 26°C on lawns of human and/or fish pathogens. The results are shown for triplicate readings and were rounded to the nearest 0.5 mm. Distances were measured from the outer portion of the P. piscicida colony to the outer periphery of the zone of clearing.
A result of <0.5 indicates that an area of clearing was observed but was too small to accurately measure.