TABLE 1.
Examples of whole-chromosome copy number variations acquired during laboratory evolution experiments with Saccharomyces cerevisiae strainsa
| Selected phenotype | Aneuploid chromosome(s) | Confirmed causality | Contributing gene(s) | Reference |
|---|---|---|---|---|
| Biomass sedimentation | Whole-genome duplication | Yes | ACE2 | 105 |
| Glucose-limited growth | Whole-genome duplication | Yes | 107 | |
| High temp tolerance | III (+1) | Yes | 17 individual genes | 103 |
| High pH tolerance | V (+1) | Yes | 103 | |
| Glucose-limited growth | I (+1), III (+1), V (+1) | No | 108 | |
| Phosphate-limited growth | IV (+1), VI (+1), X (+1), XIII (+2), XVI (+1) | No | 108 | |
| Lactate utilization by jen1Δ strain | III (+1) | Yes | ADY2 | 112 |
| Xylose utilization | I (−1) | No | 160 | |
| p-Coumaric and ferulic acid tolerance | XIV (+1) | No | 160 | |
| Copper tolerance | II (+1), VIII (+1) | No | CUP1, SCO1, and SCO2 | 104 |
| Galactose tolerance | VIII (+1) | Yes | GAL80 | 161 |
| Ethanol tolerance | III (+1), VIII (+1) | No | 106 | |
| Radicicol resistance | XV (+1) | Yes | STI1 and PDR5 | 113 |
| Fluconazole resistance | VIII (+1) | No | ERG11 | 113 |
| Tunicamycin resistance | XVI (−1) | Yes | 113 | |
| Benomyl resistance | XII (−1) | No | 113 | |
| Suppressors of MEC1 deficiency | IV (+1) | Yes | RNR1 | 162 |
| Suppressors of MYO1 deletion | XIII (+1), XVI (+1) | Yes | HSP82, HSC82, RLM1, and MKK2 | 94 |
| Suppressors of RPS24A and RNR1 deletion | IX (+1) | No | RPS24B and RNR3 | 163 |
| Suppressors of telomerase insufficiency | VIII (−1) | No | PRP8, UTP9, KOG1, and SCH9 | 164 |
a
In the examples listed, the acquired CCNV was hypothesized to contribute to the selected phenotype. “Confirmed causality” indicates that a causal link between CCNV and the phenotype acquired during laboratory evolution was experimentally confirmed. In cases where the impact of a CCNV on phenotype was linked to specific genes, this is also indicated. Segmental aneuploidies observed in the cited studies are not included in the table.