FIG 6.
AOM with AQDS as electron acceptor by an enrichment derived from wetland sediment. (a) Kinetics of methane consumption linked to AQDS reduction (to AH2QDS) observed during the last 11 days of the entire enrichment process lasting 151 days: filled squares (■) represent microcosms with CH4 as an electron donor and AQDS as an electron acceptor (complete experiments, n = 3), open squares (□) represent controls without an electron acceptor provided (without AQDS control, n = 3), filled circles (●) represent CH4-free microcosms (endogenous controls, n = 3), and crosses (×) represent heat-killed controls (sterile controls, n = 2). Error bars represent the standard error among replicates. (b and c) Microbial community changes at the end of the enrichment (151 days of incubation) at the phylum level based on Illumina sequencing of 16S rRNA V3 to V4 region. Fresh sediment composition was used as a reference.