Skip to main content
. 2004 Dec 23;102(1):163–168. doi: 10.1073/pnas.0407896101

Fig. 1.

Fig. 1.

Diagrams of the MSS116myc, cyt-19, and chimeric MSS116/CYT-19 myc genes. MSS116myc encodes the 664-aa MSS116 ORF (open box) with a 36-aa mt targeting sequence and a C-terminal myc tag. The cyt-19 cDNA (hatched box) encodes the 626-aa cyt-19 ORF with a putative 53-aa mt targeting sequence. Both Mss116p and CYT-19 contain a conserved core with nine motifs characteristic of the DEAD-box subfamily of DExH/D-box proteins (black bars, labeled for MSS116 myc). Percent similarity is shown for indicated regions of MSS116p and CYT-19. The chimeric MSS116/cyt-19 myc gene consists of the promoter and mt targeting sequence of MSS116 fused to cyt-19 codons 54–626 with a C-terminal myc tag, and the MSS116 3′ UTR. Asterisks indicate the locations of MSS116 mutations analyzed in this work.