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. 2004 Dec 23;102(1):163–168. doi: 10.1073/pnas.0407896101

Fig. 2.

Fig. 2.

Splicing phenotypes of yeast strains containing different MSS116 and cyt-19 alleles. (A–G) Northern hybridizations of mt RNAs from 30°C-grown strains containing the following mtDNA introns: aI1 (A), aI2 (B), aI5γ (C), bI1 (D), ω (E), aI3 and aI4 (F), and bI4 and bI5 (G). The blots were hybridized with 32P-labeled probes complementary to COX1 exon 6 (A–C and F), COB exon 6 (D and G), and 21S rRNA exon 2 (E). (H–J) Northern hybridizations of mt RNAs from MSS116 and mss116Δ strains with ρ- petite mtDNAs containing a 21S rRNA gene with the ω intron (H), a COX1 gene with aI5γ (I), and a COB gene with bI1 (J). Spliced mRNAs and unspliced precursor RNAs are indicated by filled and open triangles, respectively. Multiple precursor RNAs containing different combinations of introns are present in F and G. Arrows in B, C, F, and H indicate precursor RNAs with 3′ extensions. The asterisk in B indicates an aI2 splicing intermediate containing the intron and downstream exons.