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. 2004 Dec 23;102(1):163–168. doi: 10.1073/pnas.0407896101

Fig. 4.

Fig. 4.

Expression of CYT-19myc in S. cerevisiae and inability of the CYT-19 K125E mutant to support RNA splicing. (A) CYT-19myc expression. A Western blot of whole-cell protein (≈20 μg) from the indicated strains grown at 30°C was divided at the position of 40-kDa marker, and the top and bottom halves were probed with anti-myc and anti-porin antibodies, respectively. (B) The CYT-19 K125E does not support splicing of COX1 introns. Northern hybridization was carried out with RNAs from strains grown at 30°C, which have a COX1 gene containing aI3, aI4, and aI5γ and carry CEN plasmids with the nuclear genes indicated in the figure. The blot was hybridized with a 32P-labeled probe complementary to COX1 exon 6.