TABLE 2.

Oligonucleotides used for amplification and sequencing of New Zealand RCV Gore-425 strains

Primer	Sequence 5′ to 3′	Orientation	Positionsa	Reference or source
For virus detection
Rab1b	CAGCDSGCACTGCYACCACAGCATC	+	5309–5333	11
Rab2	GAAKCKRAACTGCATGCCACCAGCCCA	−	5608–5634	11
For cDNA synthesis and amplification of RCV Gore-425 VP60 capsid
Oligo dTV		+
MICV-10	CCAAACCTAGCTACCATCTTCAACTTGTAGC	−	4355–4385	11
RCV-4	TCAARATGACAGACATYGGTTGGGT	+	4946–4970	18
ALR-6	CTCGCCAGTGGTATTATAAATCTTAACAC	−	7308–7336	11
capsRCV-A1[HindIII]	AAAAGCTTATGGAGGGCAAGGCCCGTGCAACG	+	5264–5295	18
capsRCV-A1rev[NotI]	AAGCGGCCGCGTTGCTCAAAACGCCGGCACCTGC	−	7034–7076	This study
For real-time PCR
RCV RT F	GTTGGYAGGAAYGTRCCCATCATGTTTGC	+	6637–6665	18
RCV NZ R2	AGACAGGTCAATAAGTGTGGTCA	−	6896–6918	This study

^aPositions in relation to Gore-425A genome (accession no. KX357707).