FIG 4.

Host insect development and fitness after feeding with wild-type and PHA-related gene-depleted mutants of Burkholderia. (A) Survival rate was estimated each day after hatching (n = 80) by counting dead insects. (B) Adult emergence rate was observed by counting newly molted adult insects from late-fifth-instar nymphs (n = 50). (C to F) Parameters of host insect fitness. Body weight and length were measured after killing insects with acetone and drying completely in a 70°C oven (n = 40). Body weight of adult males (C), body length of adult males (D), body weight of adult females (E), and body length of adult females (F) are shown. Asterisks indicate a significant difference compared to the wild-type-infected group (ns, nonsignificant; **, P < 0.005; ***, P < 0.0005; ****, P < 0.0001 [one-way ANOVA with Tukey's correction]).