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. 2017 May 17;83(11):e00459-17. doi: 10.1128/AEM.00459-17

TABLE 2.

Primers used for cloning PCR

PCR target region Primer name Product size (bp) Sequence (5′ to 3′) Restriction site
5′ region of phaP1 phaP1-LF 1,133 ACACCAGGATCCCGCTAGGCTCTGCGAAATCT BamHI
phaP1-LR CACCTATCTAGAAACTTCAAGCAAGCCACCGA XbaI
3′ region of phaP1 phaP1-RF 1,121 ACACCATCTAGAGGGTTGCTTACACCCTCGTT XbaI
phaP1-RR CACCTAAAGCTTAATGTCCCGGTAGAGATGCG HindIII
5′ region of phaP2 phaP2-LF 1,074 ACACCAGGATCCTGTTTAGCTGGCTGCTGTTC BamHI
phaP2-LR CACCTATCTAGATCTCGAAGCTCGACACAGTT XbaI
3′ region of phaP2 phaP2-RF 1,001 ACACCATCTAGAAACTCGGCGTACGAAGCTG XbaI
phaP2-RR CACCTAAAGCTTGAAGCGTATCGACGGGGAAG HindIII
5′ region of phaP3 phaP3-LF 1,015 ACACCAGGATCCCTGTGCCTGAGGCGAGTACG BamHI
phaP3-LR CACCTATCTAGAGAAGCCTATGCGCTCGTCA XbaI
3′ region of phaP3 phaP3-RF 1,040 ACACCATCTAGACGAACCCGGGAAGCTGATAC XbaI
phaP3-RR CACCTAAAGCTTAGCGTCCCGTCCATTGAATC HindIII
5′ region of phaP4 phaP4-LF 1,018 ACACCAGGATCCGAGCCGACTCATTTCCACGA BamHI
phaP4-LR CACCTATCTAGACGGAATGAAGCAAGCGTTCG XbaI
3′ region of phaP4 phaP4-RF 1,025 ACACCATCTAGACGGTCTTGATGGTCTGCACG XbaI
phaP4-RR CACCTAAAGCTTGGAAGAGTCGCCTGTCGAG HindIII
5′ region of phaR phaR-LF 1,027 ACACCAGGATCCCGACGAGATCGGTCATGCG BamHI
phaR-LR CACCTATCTAGAGCAGGCGAAGTCGATGTTCA XbaI
3′ region of phaR phaR-RF 1,036 ACACCATCTAGACGTGATGTACGTGCTCGTCT XbaI
phaR-RR CACCTAAAGCTTGAGATCGCCGTCCGCATAA HindIII
Complementation of phaR phaR-comF 1,232 ACACCAGAATTCGCGAAACCATGCCGATTTTC EcoRI
phaR-comR CACCTAGAATTCGGACATGGTGAAGTCCATCC EcoRI