TABLE 2.
Primers used for cloning PCR
| PCR target region | Primer name | Product size (bp) | Sequence (5′ to 3′) | Restriction site |
|---|---|---|---|---|
| 5′ region of phaP1 | phaP1-LF | 1,133 | ACACCAGGATCCCGCTAGGCTCTGCGAAATCT | BamHI |
| phaP1-LR | CACCTATCTAGAAACTTCAAGCAAGCCACCGA | XbaI | ||
| 3′ region of phaP1 | phaP1-RF | 1,121 | ACACCATCTAGAGGGTTGCTTACACCCTCGTT | XbaI |
| phaP1-RR | CACCTAAAGCTTAATGTCCCGGTAGAGATGCG | HindIII | ||
| 5′ region of phaP2 | phaP2-LF | 1,074 | ACACCAGGATCCTGTTTAGCTGGCTGCTGTTC | BamHI |
| phaP2-LR | CACCTATCTAGATCTCGAAGCTCGACACAGTT | XbaI | ||
| 3′ region of phaP2 | phaP2-RF | 1,001 | ACACCATCTAGAAACTCGGCGTACGAAGCTG | XbaI |
| phaP2-RR | CACCTAAAGCTTGAAGCGTATCGACGGGGAAG | HindIII | ||
| 5′ region of phaP3 | phaP3-LF | 1,015 | ACACCAGGATCCCTGTGCCTGAGGCGAGTACG | BamHI |
| phaP3-LR | CACCTATCTAGAGAAGCCTATGCGCTCGTCA | XbaI | ||
| 3′ region of phaP3 | phaP3-RF | 1,040 | ACACCATCTAGACGAACCCGGGAAGCTGATAC | XbaI |
| phaP3-RR | CACCTAAAGCTTAGCGTCCCGTCCATTGAATC | HindIII | ||
| 5′ region of phaP4 | phaP4-LF | 1,018 | ACACCAGGATCCGAGCCGACTCATTTCCACGA | BamHI |
| phaP4-LR | CACCTATCTAGACGGAATGAAGCAAGCGTTCG | XbaI | ||
| 3′ region of phaP4 | phaP4-RF | 1,025 | ACACCATCTAGACGGTCTTGATGGTCTGCACG | XbaI |
| phaP4-RR | CACCTAAAGCTTGGAAGAGTCGCCTGTCGAG | HindIII | ||
| 5′ region of phaR | phaR-LF | 1,027 | ACACCAGGATCCCGACGAGATCGGTCATGCG | BamHI |
| phaR-LR | CACCTATCTAGAGCAGGCGAAGTCGATGTTCA | XbaI | ||
| 3′ region of phaR | phaR-RF | 1,036 | ACACCATCTAGACGTGATGTACGTGCTCGTCT | XbaI |
| phaR-RR | CACCTAAAGCTTGAGATCGCCGTCCGCATAA | HindIII | ||
| Complementation of phaR | phaR-comF | 1,232 | ACACCAGAATTCGCGAAACCATGCCGATTTTC | EcoRI |
| phaR-comR | CACCTAGAATTCGGACATGGTGAAGTCCATCC | EcoRI |