TABLE 1.

Primer, probe, and inhibition control synthetic gene sequences

Primer, probe, or control gene name	Sequence (5′–3′)a
E. vermicularis 5S_fwd	CAAACAACTGCATCACCAATAAC
E. vermicularis 5S_rev	AGTGTAGAGCAATAAGCAGTAAAG
E. vermicularis 5S probe	FAM-TACCAACAACACTTGCACGTCTCTTCA-IBFQ
E. caproni 28S_fwd	TAGGCAATGTGGTGTTCAGG
E. caproni 28S_rev	CATAGAGGGTGAAAGGCCC
E. caproni 28S probe	FAM-AGGACTTAGGGTGGAGCAGTATCCC-IBFQ
Inhib Con_fwd	GGTTACGTTACCGATTGTGTTAT
Inhib Con_rev	GCACGGAGAAGTATTCTTGATATAG
Inhibition control probe	JOE-TCGCATACTATGACCGAGACCCTGT-IBFQ
Inhibition control sequence	CCAATGCAATGGCTAACACAATATTATATTTTAAAGAATGAATATCATAAGACCATGCTAGCGTATGATAATGGATCTTGGACATAAGATATAGTTCTTATGAAGAGGCAAAAAAACACGATGATGGACTTATCATTATTTCTATATTCTTACAAGTATTGGATCATAA

^aFAM, 6-carboxyfluorescein; IBFQ, Iowa black fluorescent quencher; JOE, 6′-carboxy-4′,5′-dichloro-2′,7′-dimethoxyfluorescein, succinimidyl ester.