Fig. 6.

Loss of MECR, Mpv17, and MnSOD affect HIF-1α protein stability. (A,C,E) NIH 3T3-scr, MECR-KD, HepG2-scr, MnSOD-KD, Mpv17^+/+, and Mpv17^-/- cells were cultured for 20 h under hypoxia (1% O₂). After inhibition of protein synthesis with cycloheximide (CHX; 10 µg/ml) HIF-1α protein levels were measured by Western blot analysis at the indicated time points. The HIF-1α protein levels without CHX were set to 1. *, significant difference, p≤0.05. (B,D,F) Representative immunoblots. One hundred micrograms of total protein were analyzed with antibodies against HIF-1α and α-tubulin.