Table 1.
Cell Line | Gene | Sequence |
---|---|---|
SCC74A | human IFNα sense | 5′-AGCCATCTCTGTCCTCCATGAG-3′ |
human IFNα anti-sense | 5′-TGCATCACACAGGCTTCCAA-3′ | |
human MCP1 sense | 5′-ATCTCCTTGGCCACAATGGTC-3′ | |
human MCP1 anti-sense | 5′-AGATGCAATCAATGCCCCAG-3′ | |
human GAPDH sense | 5′-GGAGTCAACGGATTTGGTCG-3′ | |
human GAPDH anti-sense | 5′-GGAATCATATTGGAACATGTAAACC-3′ | |
MTE | murine IFNα sense | 5′-CCTGCTGGCTGTGAGGAAATA-3′ |
murine IFNα anti-sense | 5′-TCTCAGTCTTCCCAGCACATTG-3′ | |
murine MCP1 sense | 5′-AACCTGGATCGGAACCAAATG-3′ | |
murine MCP1 anti-sense | 5′-GCTTGAGGTGGTTGTGGAAAAG-3′ | |
murine GAPDH sense | 5′-AGCCTCGTCCCGTAGACAAAAT-3′ | |
murine GAPDH anti-sense | 5′-GAAGACACCAGTAGACTCCACGACAT-3′ |
Primer list for real-time continuous detection of PCR products using SYBR Green. GAPDH was used as an internal control. IFN, interferon; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; MCP, monocyte chemotactic protein.