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. 2017 May 10;5:87–96. doi: 10.1016/j.omto.2017.05.002

Table 1.

Primer List for Gene Expression Analysis in Human SCC74A and MTE Cell Lines

Cell Line Gene Sequence
SCC74A human IFNα sense 5′-AGCCATCTCTGTCCTCCATGAG-3′
human IFNα anti-sense 5′-TGCATCACACAGGCTTCCAA-3′
human MCP1 sense 5′-ATCTCCTTGGCCACAATGGTC-3′
human MCP1 anti-sense 5′-AGATGCAATCAATGCCCCAG-3′
human GAPDH sense 5′-GGAGTCAACGGATTTGGTCG-3′
human GAPDH anti-sense 5′-GGAATCATATTGGAACATGTAAACC-3′
MTE murine IFNα sense 5′-CCTGCTGGCTGTGAGGAAATA-3′
murine IFNα anti-sense 5′-TCTCAGTCTTCCCAGCACATTG-3′
murine MCP1 sense 5′-AACCTGGATCGGAACCAAATG-3′
murine MCP1 anti-sense 5′-GCTTGAGGTGGTTGTGGAAAAG-3′
murine GAPDH sense 5′-AGCCTCGTCCCGTAGACAAAAT-3′
murine GAPDH anti-sense 5′-GAAGACACCAGTAGACTCCACGACAT-3′

Primer list for real-time continuous detection of PCR products using SYBR Green. GAPDH was used as an internal control. IFN, interferon; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; MCP, monocyte chemotactic protein.