Table 2. Metabolomic Determinations^a in Glucose-Grown Pseudomonas putida KT2440 Carrying Glycolytic Genes Borne by Module I.

	intracellular content (nmol mg CDW–1) of
P. putida KT2440 carrying plasmid	glucose-6-P	glyceraldehyde-3-P	NADPH
pSEVA224 (empty vector)	52 ± 9	0.39 ± 0.08	43 ± 5
pS224·GBI (Module I)	89 ± 4	1.23 ± 0.07	32 ± 9

^aCells were grown aerobically in M9 minimal medium added with glucose at 20 mM, harvested during exponential growth, and rapidly quenched with liquid N₂. The intracellular metabolites were extracted and their concentration determined by means of liquid chromatography coupled to mass spectrometry. Each parameter is reported as the mean value ± standard deviation from duplicate measurements in at least two independent experiments.