TABLE 6.

Infection of U87 cells

Virus	GHOSTb	RT concna
		Parental	CCR1	CCR2b	CCR3	CCR5	CXCR4	CXCR6
Aviremic individuals
RH2-3 3B3	R5G15X6	15,274	3,795	216	3,946	16,092	3,059	6,143
RH2-3 3C3	R5G15X6	31,055	4,965	477	>31,055	>31,055	7,683	16,701
RH2-3 5B1	R5G15X6	7,394	1,718	324	5,876	>31,055	1,829	1,272
RH2-3 8A5	R5G15X6	>31,055	2,013	303	2,823	17,001	1,834	10,598
RH2-3 8A3	R5G15X6	13,886	3,523	202	6,105	22,182	3,071	2,008
RH2-13 1D6	R5G15X6	>31,055	3,363	341	21,154	27,143	>31,055	1,859
RH2-13 5C1	R5G15X6	>31,055	3,595	116	8,169	>31,055	16,336	2,927
RH2-13 7C3	R5G15X6	27,631	826	100	143	26,904	678	1,959
RH2-13 6A2	R5G15X6p	>31,055	4,827	212	10,103	>31,055	18,746	1,387
RH2-13 6B1	R5G15X6p	−	−	−	−	>31,055	NT	11
RH2-14 1C3	R5G15X6	1,444	1,426	100	498	29,452	519	9,996
RH2-14 1D1	R5G15X6	5,484	865	113	431	9,902	675	13,341
RH2-14 2A2	R5G15X6	4,165	3,529	389	639	>31,055	448	>31,055
RH2-14 2A5	R5G15X6	4,867	3,582	156	823	27,631	330	9,098
RH2-14 2D5	R5G15X6	1,921	646	103	71	18,281	102	9,809
RH2-22 2B2	R5G15X6	617	42	−	19	14,658	16	1,302
RH2-22 2C4	R5G15X6	365	33	13	−	8,349	39	1,323
RH2-22 3A1	R5G15X6	226	−	−	11	19,186	16	310
RH2-22 2A4	R5G15X6p	639	39	15	32	>31,055	68	4,304
RH2-22 1B1	R1R5G15X6p	438	71	19	45	7,652	68	1,649
RH2-22 1B2	R1R5G15X6p	555	26	−	11	10,837	21	2,152
RH2-22 2C2	R5G15X6p	166	29	16	49	5,829	28	724
RH2-8 3D5	R5G15X6p	48	16	−	−	12,388	−	52
RH2-4 3B1	R5G15X6p	6,806	1,255	449	1,168	20,467	962	15,786
RH2-4 4D1	R5G15X6	758	375	107	273	27,083	131	3,988
Viremic individuals
Asymptomatic
RH2-15 B9	R5G15X6	81	12	−	−	11,685	11	888
RH2-15 E5	R5G15	52	−	−	−	21,404	−	252
RH2-15 A9	−	56	−	19	−	31	−	48
RH2-15 E10	R3R5G15X6	39	162	42	18,450	15,117	20,669	14,314
Progressor
RH2-1 A9	R5G15	136	21	−	−	25,645	−	100
RH2-1 C7	R5	892	31	−	−	17,690	−	−
RH2-1 D9	R5G15	42	13	−	10	10,700	−	30
RH2-1 G7	R5G15	6,585	162	−	18	17,786	176	527
RH2-5 2D11	R5G15X6	9,793	−	−	−	>31,055	−	661
RH2-5 2A12	R5G15X6	927	42	37	−	24,838	212	324
RH2-5 2F10	R5G15X6p	30,078	10,889	206	20,467	15,301	1,379	11,875
RH2-7 E4	R5G15X6	19,935	107	10	−	9,809	220	9,809
RH2-7 B3	R5G15X6p	27,385	24,733	2,208	14,509	23,918	25,380	>31,055

^aShown are concentrations of RT (pg/ ml) in culture supernatants after 14 days of culture. Negative cutoff of the assay is 10 pg/ml. Values below cutoff are considered negative (−). Values in the coreceptor-transduced cell lines that are at least 1.5 times higher than values in parental cells are underlined. NT, not tested.

^bCoreceptor usage in GHOST cells as deduced from tables 3 and 5. R1, CCR1; R5, CCR5; G15, GPR15; X6, CXCR6; p, parental infection. Note that GPR15 was not tested in U87 cells.