Figure 1. TCR binds pMHC with high affinity and peptide specificity.

(a,b) SPR analyses of B4.2.3 binding to P18-I10/H2-D^d (a) or to MTF/H2-D^d (b). B4.2.3 was offered to immobilized P18-I10/H2-D^d complexes at concentrations of 0.16, 0.32, 0.62, 1.25, 2.5 and 5.0 μM and data were analysed as detailed in the Methods. Inset to a shows binding isotherm with indicated fitted parameters in the top of the plot. (c,d) Sedimentation velocity analytical ultracentrifugation (SV-AUC) analyses of interaction of B4.2.3 TCR with P18-I10/H2-D^d (c) or with MTF/H2-D^d (d). Centrifugation and analysis are described in the Methods.