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. 2017 May 16;8:15261. doi: 10.1038/ncomms15261

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Copyright © 2017, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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(a) Light microscopy image of 3βHSD expression (purple) along edge of follicle cultured in 30° scaffold after 4 days of culture. (b) Estradiol secretion in media of follicles cultured in 3D printed scaffolds collected at day 2 and day 8 of culture. (c,d) MII egg with extruded polar body was released from a follicle cultured in 60° scaffold and contains condensed chromatin (blue) along the spindle (green). (e,f) Scanning electron micrograph demonstrating follicle (arrows) wedged underneath three layers of 60° scaffold struts (identified as layers A, B, C) and cultured for 2 days. Scale bars: (c) 50 μm; (a,e) 100 μm; (d,f) 10 μm.