Table 1. Description of three experimental designs used to investigate ovarian follicles in the 3D printed scaffolds.

Experiment	Follicle size	Scaffold size, angle	Duration (mice per group)
(a) In vitro: to test effects of architecture on survival to stimulate in vitro maturation	3–4 150–180 μm follicles per scaffold to visualize health of each under light microscopy	3–2 mm30°, 60°, 90°	2–8 days
(b) In vivo: to test vascularization and hormone restoration	Day 1: primordial, primary and small secondary follicles; Day 2: filled in with more follicles ≤180 μm; 40–50 follicles total	2 mm (to fit in bursa)60°	1 or 3 weeks post-surgery(16 OVX+implant; 5 OVX)
(c) In vivo: to test restorative organ function—mating, ovulation, live birth, lactation	Day 1: primordial, primary and small secondary follicles; Day 2: filled in with more follicles ≤180 μm; 40–50 follicles total	2 mm (to fit in bursa)60°	8–10 weeks post-surgery(7 OVX+implant; 2 OVX)

Ovariectomized animals with ovarian bioprosthesis implant are labelled as ‘OVX+implant'. Ovariectomized animals with a sham implant (3DP scaffold without cells) are labelled as ‘OVX'.