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. 2017 May 16;8:15287. doi: 10.1038/ncomms15287

Figure 6. Reduction of ROS levels attenuated the effects of Alix or Rab27a knockdown in HDFs.

Figure 6

Pre-senescent TIG-3 cells were transfected with validated siRNA oligos indicated at the top of the panel for two times at 2 day intervals in the presence or absence of 1 mM N-acetyl cysteine. These cells were then subjected to western blotting using antibodies shown right (a), analysis of intracellular ROS levels (b), immunofluorescence staining for markers of DNA damage (γ-H2AX (red), pST/Q (green) and 4′,6-diamidino-2-phenylindole (blue)) (c) or to apoptosis analysis (d). The histograms indicate the percentage of nuclei that contain more than 3 foci positive for both γ-H2AX and pST/Q staining (c). At least 100 cells were scored per group (c). The representative data from three independent experiments are shown. For all graphs, error bars indicate mean±s.d. of triplicate measurements. (*P<0.05. **P<0.01. ***P<0.001; one-way analysis of variance).