FIG. 10.

LMP2A enhances global tyrosine phosphorylation of cellular proteins in response to adhesion. Neo control and LMP2A- and LMP2B-expressing SCC12F cells were serum starved, collected as single cells, and held in suspension for 60 min prior to plating onto fibronectin-coated petri dishes. At various times after plating, cells were harvested and subjected to immunoblotting with an antibody to (A) p-Tyr (4G10) to assess the degree of tyrosine phosphorylation and (B) β-actin to ensure equal loading.