FIG. 11.

LMP2A but not LMP2B induces constitutive Akt phosphorylation in the absence of growth factor and integrin signals. (A) Neo control and LMP2A- and LMP2B-expressing SCC12F cells were serum starved, collected as single cells, and held in suspension for 60 min in the absence of serum. The degree of Akt phosphorylation was assessed after immunoblotting total cell lysates with an antibody recognizing the serine 473-phosphorylated form of active Akt. (B) The same cells were then plated onto fibronectin-coated dishes and harvested for immunoblotting at the indicated times. Total cell lysates were assayed for Akt phosphorylation using an antibody recognizing the serine 473-phosphorylated form of active Akt. The same blots were then stripped and reprobed with antibodies for total Akt and β-actin to ensure equal loading.